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RE: Methods, Methods, Methods (My Research, Vol. 5)
Nice job! A few things to remember:
- Run to red
- You can check your gel tray for little bubbles to make sure stuff is hooked up OK
- EtBR is less toxic than we thought, but if EH&S is giving you issues, there are alternative dyes (GelRed)
PCR tips:
https://theupturnedmicroscope.com/tag/pcr/
https://goo.gl/images/2UQYW3
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suesa (72) 7 years ago
- We have everything color-labeled because of this
- Never leave the lab without checking for bubbles
- I don't exactly have a choice what to use ^^