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RE: Gene Editing - Cre/loxp (My Research, Vol. 2)
Very interesting how you explained this technique! The Cre/loxp system is a great method that has been used for many years and as you poninted out it is much more precise compared to newer modification methods such as CRISPR/Cas9. What do you think about its handling? I think in this regard CRISPR is advantageous. There is actually a modification of the CRISPR/Cas9 system called CRISPRi/a which does not edit the genes itself but modifies the gene regulation. I recently wrote about it, maybe you like to check it out.
I've just read three papers and a 125 pages ebook about crispr, I'm not sure if I can take it anymore 😅
@schay and @suesa send me any CRISPR/Cas9 questions you have. I use it every day to make new human stem cell lines.
Good to know! I'll be using it to create a loxp site in a specific gene of mice. I'll message you when it doesn't work :D
Wow! That is quite some research on CRISPR. I can imagine, you had enough.