@suesa : I loved this post very much.

The person who had built this construct (another student, apparently) had managed to build a plasmid without marker gene and thus ruined 7 weeks of work for me.

So sad. Working with a group of people is good and bad. Sometimes it gives your great success, sometimes you struggle. I can understand how difficult it would be in a wet lab compared to a computational lab. By the way I thought you are an undergrad. (Or Am I wrong?) To get these experiences in the early stage of your career is great.