PCR

in #molecularbiology7 years ago

PCR (Polymerase Chain Technology) is a technique used in molecular biology to amplify a single copy of a particular sequence of DNA to millions of Copies.

IMAG0360.jpg

The mix required is prepared in a single tube and is subjected to the virtual DNA Amplification.

IMAG0352.jpg

The majority of the techniques relies on a thermal cycling which involves series of heating and cooling. The steps includes:

Polymerase_chain_reaction.svg.png

Denaturation:
The double stranded DNA is subjected to heat up to 94-96 degrees in order to separate the strands.

Annealing:
The each strands are annealed with Taq (Thermal Aquaticus) polymerase in temperature 50 to 65 degrees.

Extension:
In this step the polymerase synthesizes a complimentary DNA at temperature 75-80 degrees.

PCR is used in:

a. Viral Loads Quantification(eg: HCV, HIV, HBV, CMV,etc)
b. Forensic Applications
c. Genetic Testing
d. Oncogenesis
e. Researches

Sort:  

Nice post! I will follow you from now on. +UP

Congratulations @naiadsus! You have completed some achievement on Steemit and have been rewarded with new badge(s) :

Award for the number of upvotes

Click on any badge to view your own Board of Honor on SteemitBoard.
For more information about SteemitBoard, click here

If you no longer want to receive notifications, reply to this comment with the word STOP

Upvote this notification to help all Steemit users. Learn why here!

Congratulations @naiadsus! You have received a personal award!

1 Year on Steemit
Click on the badge to view your Board of Honor.

Do not miss the last post from @steemitboard!


Participate in the SteemitBoard World Cup Contest!
Collect World Cup badges and win free SBD
Support the Gold Sponsors of the contest: @good-karma and @lukestokes


Do you like SteemitBoard's project? Then Vote for its witness and get one more award!

Congratulations @naiadsus! You received a personal award!

Happy Birthday! - You are on the Steem blockchain for 2 years!

You can view your badges on your Steem Board and compare to others on the Steem Ranking

Vote for @Steemitboard as a witness to get one more award and increased upvotes!

Coin Marketplace

STEEM 0.18
TRX 0.16
JST 0.029
BTC 76065.61
ETH 2908.14
USDT 1.00
SBD 2.59